One-tube method for complete HPA-1 genotyping by PCR using sequence-specific primers
نویسندگان
چکیده
منابع مشابه
Establishment of an optimized PCR method using sequence-specific primers for screening multiple gene polymorphisms simultaneously.
The present study aimed to explore the factors that affect polymerase chain reaction using sequence-specific primers (PCR-SSP) and to establish an optimized PCR-SSP method for detecting multiple gene polymorphisms simultaneously. The amplification system parameters, including the concentrations of Mg2+, dNTPs, pfu Taq, primers and control primers, were optimized using the designed PCR-SSP react...
متن کاملHigh-throughput SNP genotyping by single-tube PCR with Tm-shift primers.
Despite many recent advances in high-throughput single nucleotide polymorphism (SNP) genotyping technologies, there is still a great need for inexpensive and flexible methods with a reasonable throughput. Here we report substantial modifications and improvements to an existing homogenous allele-specific PCR-based SNP genotyping method, making it an attractive new option for researchers engaging...
متن کاملRapid and specific genotyping system for hepatitis B virus corresponding to six major genotypes by PCR using type-specific primers.
A simple and precise genotyping system based on PCR using type-specific primers was developed for the determination of genotypes A through F of hepatitis B virus (HBV). This assay system is considered to be a useful tool for the molecular diagnosis of HBV infection and for large-scale surveys.
متن کاملABO glycosyltransferase genotyping by polymerase chain reaction using sequence-specific primers.
Serological typing for the classical ABO blood groups is routinely performed using anti-A and anti-B antisera of polyclonal or monoclonal origin, which are able to distinguish four phenotypes (A, B, AB, and O). Modern molecular biology methods offer the possibility of direct ABO genotyping without the need for family investigations. Typing can be done with small amounts of DNA and without detec...
متن کاملHigh-throughput SNP genotyping by allele-specific PCR with universal energy-transfer-labeled primers.
We have developed a new method for high-throughput genotyping of single nucleotide polymorphisms (SNPs). The technique involves PCR amplification of genomic DNA with two tailed allele-specific primers that introduce priming sites for universal energy-transfer-labeled primers. The output of red and green light is conveniently scored using a fluorescence plate reader. The new method, which was va...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: British Journal of Haematology
سال: 1997
ISSN: 0007-1048
DOI: 10.1046/j.1365-2141.1997.5013303.x